Circular RNA validation based on sequencing of RNase R treated/untreated samples and details of circRNA chr2: 58,311,224|58,316,858. (A) Overlap of prediction results between two samples (RNaseR+, ribominus RNA treated with RNase R; RNaseR-, ribominus RNA). (B) Coverage of five exons contained in chr2: 58,311,224|58,316,858 in the two samples (red, junction reads identified by CIRI in RNaseR- sample; blue, junction reads identified by CIRI in RNaseR+ sample; grey, other reads). Scissors indicate the splicing sites, which are flanked by GT-AG signals highlighted in red. (C) circRNA structure and its linear amplified fragment using a pair of outward-facing primers. (D) Sequencing chromatogram of the PCR product across the junction.