Comparison of TopHat-Fusion with TRUP analysis tools. The fusion candidates found by TRUP and TopHat-Fusion on sample S00054, plotted by the number of non-redundant spanning reads reported by the two tools. The horizontal and vertical dashed lines indicate the threshold of three spanning reads for calling a chimeric transcript. The diagonal dashed line is plotted to show that TRUP usually reports more reads spanning a fusion point. The axes are in log2 scale. The unique calls to each algorithm are jittered to avoid over-plotting.