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Figure 5 | Genome Biology

Figure 5

From: Determining the quality and complexity of next-generation sequencing data without a reference genome

Figure 5

Detecting the balance in coverage depth of plus and minus strands in sequencing data. (A) Scatter plot of distance between the frequencies of k-mers and their reverse complement (balance) versus the total number of reads in WGS data. The poly2 fitted line and the 95% confidence intervals are indicated. (B) Scatter plot of balance versus the total number of reads in WES data. The red circle indicates an outlier with an extreme duplication rate and imbalance of coverage between the plus and minus strands. (C) Scatter plot of balance versus the total number of reads in RNA-Seq data. RNA-Seq, RNA sequencing; WES, whole exome sequencing; WGS, whole genome sequencing.

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