MAGeCK is robust to the number of targeting sgRNAs per gene. This figure shows the effect of different numbers of targeting sgRNAs per gene. Each bar indicates the percentage of top-ranked, 'reference' genes that are identified by MAGeCK, RIGER and RSA using different numbers of sgRNAs per gene. 'Reference' genes are those that are in the top 5% of ranked genes in all three methods when using 10 sgRNAs per gene. See Materials and methods for sampling details. Error bars represent the standard deviation from three independent sampling experiments.