Figure 1
From: Transcriptome-wide modulation of splicing by the exon junction complex
Identification and validation of splicing changes in EJC KD cells. (A) Venn diagrams showing the overlap between significant splicing events (cassette exons) identified with MISO and DiffSplice (left panels), using a threshold of 0.1 for ΔΨ. Correlation between the ΔΨ values predicted by MISO and DiffSplice are shown on the right, and Pearson’s correlation coefficient (r2) is indicated. (B,C) RT-PCR validation of two alternative exons (B) and one constitutive exon (C) in EJC and Upf1 KD cells. MRPL3 is predicted to increase in exon skipping (ΔΨ >0.1) in EJC KD whereas C20orf7 is predicted to increase in exon inclusion (ΔΨ <0.1) in EJC KD. KPNA1 is predicted to increase in exon skipping (ΔΨ >0.1) in EJC KD. The quantification of triplicate experiments are shown below as mean ± SD. ***P <0.001, one-way ANOVA.