Skip to main content
Figure 7 | Genome Biology

Figure 7

From: Ontogeny of CpG island methylation and specificity of DNMT3 methyltransferases during embryonic development in the mouse

Figure 7

Role of DNMT3B at imprinted differentially methylated regions and X-linked CpG islands in embryos. (A) Box-plot representing the methylation of 17 imprinted germline DMRs in WT, Dnmt3a-/- and Dnmt3b-/- E8.5 embryos. (B) Dynamics of methylation throughout development and in Dnmt3 knockout E8.5 embryos at somatic DMRs: Cdkn1c (chr7:143,459,734-143,460,383), Slc22a18 (chr7:143,465,018-143,465,543), Meg3 (chr12:109,540,809-109,541,073), H19 (chr7:142,578,145-142,578,462) and Mkrn3 (chr7:62,419,498-62,420,497). (C) Dynamics of methylation throughout development and in Dnmt3 knockout E8.5 embryos at the three DMRs of the Gnas locus. The genomic organization of the locus and the position of the DMRs are shown on top of the graph. The detailed RRBS profiles are shown in Figure S9B in Additional file 1. (D) Examples of RRBS profiles at CGI promoters of three X-inactivated genes in female WT and Dnmt3-mutant embryos at the E8.5 stage. (E) Box plots of the global distribution of CGI methylation in autosomes (left) and on the X chromosome (right) in WT E8.5 embryos, female Dnmt3-mutant embryos and female adult liver.

Back to article page