Figure 4From: Integrated analyses of DNA methylation and hydroxymethylation reveal tumor suppressive roles of ECM1, ATF5, and EOMESin human hepatocellular carcinomaThe effect of siRNA knockdown on hepatocellular carcinoma cell growth. (A) Validation of DNA methylation for biological replication (blue box indicate non-HCC and red box indicate HCC samples). Growth curve of YY8103 and QGY7701 cells treated with siRNA-NC, (B, C) ATF5-siRNA1 and ATF5-siRNA2, (D, E) ECM1-siRNA1 and ECM1-siRNA2, and (F) EOMES-siRNA1 and EOMES-siRNA2. The expression of genes was analyzed using both qRT-PCR and western blot.Back to article page