Interferon production and signaling in HeV-infected HEK293T cells. (A) GO terms enriched in the up-regulated genes/proteins in HEK293T cells were analyzed and visualized using REVIGO, where redundant GO terms are removed. The size of the circle represents the number of enriched child GO terms contributing to the parent term. The circle color represents the uncorrected log10 P-value for each parent GO term. Semantic space is the result of multi-dimensional scaling where similar GO terms cluster together. A full list of enriched GO terms, including false discover rate-corrected P-values are presented in Additional file 5. (B) Top 10 most over-represented KEGG pathways, and the number of significantly differentially expressed genes/proteins contributing to each pathway. The full list of over-represented KEGG pathways is presented in Additional file 6. Validation of RNAseq differential expression with real-time qPCR for (C) IFNB1, (D) IFIT2 and (E) IFIT3. Real-time PCR differential expression was calculated based on relative expression to 0 hpi and normalized to GAPDH. Log2-fold change is presented; *P <0.05, **P <0.01.