Summary of heterogeneity observed across all samples. (A) Protein-altering mutations detected across all tumor regions. Genes mutated twice in the same tumor region are not identified here but can be found within a table of all mutations (Table S3 in Additional file 1). (B) High level gene amplifications detected by smMIP assay. Copy number was estimated by comparing all tumor samples against 12_X, a universal control from BI12 (see Figure S2 in Additional file 1 for analysis using patient matched controls). ‘Amplification’ indicates genes with coverage three-fold higher than median coverage across a sample. ‘High Amplification’ indicates genes with coverage six-fold higher than median coverage across a sample. Region X refers to brain tissue grossly uninvolved by tumor. Our approach would miss any low-level gene amplifications within these tumors.