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Figure 8 | Genome Biology

Figure 8

From: Sex differences in the genome-wide DNA methylation pattern and impact on gene expression, microRNA levels and insulin secretion in human pancreatic islets

Figure 8

Technical validation of Infinium HumanMethylation450 BeadChip data using PyroSequencing. (A-C) The selected CpG site connected to NKAP (probe ID: cg27483305) (A), APLN (probe ID: cg05688478) (B) and GREB1 (probe ID: cg13808071) (C) showed similar differences in DNA methylation between males and females for both PyroSequencing and Infinium HumanMethylation450 BeadChip data. Numerous surrounding CpG sites in NKAP (A), APLN (B) and GREB1 (C) analyzed by PyroSequencing also show differential DNA methylation between male and female islet donors. Data are presented as mean ± standard error of the mean. Asterisks indicate P <0.0005 for PyroSequencing data and q <0.05 for Infinium HumanMethylation450 BeadChip data. PyroSequencing data were calculated using a Mann-Whitney test.

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Genome Biology

ISSN: 1474-760X

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