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Figure 10 | Genome Biology

Figure 10

From: Sex differences in the genome-wide DNA methylation pattern and impact on gene expression, microRNA levels and insulin secretion in human pancreatic islets

Figure 10

Impact of Nkap , Apln and Bcl11a on insulin secretion in clonal β-cells. Glucose-sensitive clonal INS-1 832/13 β-cells were used to study the impact of Nkap, Apln and Bcl11a on insulin secretion. (A-C) Transfection of clonal β-cells with siRNA targeting Nkap, Apln and Bcl11a resulted in decreased Nkap (A), Apln (B) and Bcl11a (C) mRNA expression (black bars) when compared with clonal β-cells transfected with negative control siRNA (siNC, white bar) (n = 4). *P <0.05 using a Mann-Whitney test. (D) Insulin secretion in response to 2.8 mM (white bars) and 16.7 mM (black bars) glucose in clonal β-cells deficient for Nkap (n = 10) or Apln (n = 6) expression compared with control cells transfected with negative control siRNA (siNC) (n = 10). *P <0.05. Also, basal insulin secretion at 2.8 mM glucose showed a slight increase in siNkap-treated cells compared with control (¤P = 0.049). (E) Glucose-stimulated insulin secretion presented as the ratio of secretion at 16.7 over that at 2.8 mM glucose (fold change) in clonal β-cells deficient for Nkap (n =10) or Apln (n = 6) expression (black bars) compared with control cells transfected with negative control siRNA (siNC, white bar) (n = 10). (F) Insulin secretion in response to 2.8 mM (white bars) and 16.7 mM (black bars) glucose in clonal β-cells deficient for Bcl11a (n = 9) expression compared with control cells transfected with negative control siRNA (siNC) (n = 9). *P <0.05. (G) Glucose-stimulated insulin secretion presented as the ratio of secretion at 16.7 over that at 2.8 mM glucose (fold change) in clonal β-cells deficient for Bcl11a (n = 9) expression (black bars) compared with control cells transfected with negative control siRNA (siNC, white bar) (n = 9). *P <0.05. The insulin secretion data were normalized to total protein content. Insulin secretion data were analyzed using a Wilcoxon test. Data are presented as mean ± standard error of the mean.

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