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Table 1 LASV genome coverage from standard RNA-seq and hybrid selection libraries

From: Enhanced methods for unbiased deep sequencing of Lassa and Ebola RNA viruses from clinical and biological samples

  Standard Hybrid selection
LASV sample Total reads (×10 6) Median coverage Normalized coverage a Assembled LASV genome? Total reads (×10 6) Median coverage Normalized coverage a Assembled LASV genome?
G090 5.2 1 0.28 No 1.2 20 19.25 Yes
G2230 1.3 2 7.73 No 1.2 1 24.84 No
G733 6.9 85 17.18 Yes 1.3 527 636.71 Yes
G771 24.5 65 3.55 Yes 2.5 14 12.56 Yes
ISTH0073 35.0 115 3.86 Yes 1.5 208 197.28 Yes
ISTH0230 7.3 4 0.33 No 1.3 6 4.28 Yes
ISTH1137 8.1 18 2.86 Yes 8.0 47 6.84 Yes
ISTH2020 8.9 28 5.26 Yes 1.2 53 78.84 Yes
ISTH2025 40.2 13 0.60 Yes 1.2 30 43.83 Yes
ISTH2050 6.9 20 3.44 Yes 1.2 18 41.94 Yes
LM032 14.9 121 8.99 Yes 12.3 1,003 88.18 Yes
LM222 6.3 6 0.96 Yes 2.6 390 158.73 Yes
Z002 5.8 0 0.08 No 1.1 23 26.09 Yes
  1. aAverage base coverage per 1 million reads. Successful LASV genome assembly required >1× coverage of 90% of LASV ORF covered. Coverage metrics are based upon unique, non-duplicated LASV reads. G-series: Sierra Leone clinical isolates (4). ISTH series: Nigeria clinical isolates (6). LM and Z series: Mastomys natalensis isolates. Other metrics including average (×) coverage and % genome coverage at >1× are included in Additional file 1: Table S2.