Depletion of rRNA from EBOV-Sierra Leone clinical samples. (A) Percentage rRNA (left) and unique EBOV content (right) with (gray) and without (blue) rRNA depletion in four individual clinical serum isolates (G3676-2, G3677-1, G3677-2, G3682-1). (B) Average EBOV genome coverage with (gray) and without (blue) rRNA depletion from four individual isolates with standard deviation (black). N, VP35, VP40, GP, VP30, VP24, L: boundary for each gene in the EBOV genome. Positions and variant allele of two iSNVs (in G3676-2 only) observed after rRNA depletion are depicted.