Depletion of rRNA from human LASV isolates. (A) Rarefaction analysis of LASV sample (ISTH2016) from a rRNA-depleted (gray) or control (undepleted, blue) preparation. Data best fit (dashed line) to the Michelis-Menten formula in which projected saturation value equals V
(see Materials and methods). (B) LASV genomic coverage from a LASV sample (ISTH0073) from a rRNA-depleted (gray) or control (blue) preparation. L, S segment, Z, L, NP, GPC: boundaries of each LASV genomic segment with specified genes encoded on each segment. (C) Starting overall content (RNA input) and enrichment of unique LASV (Library content) upon rRNA depletion from nine different clinical isolates.