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Table 3 Identification of Rnr genes required for NHR of barley to B. graminis f.sp. tritici

From: Discovery of genes affecting resistance of barley to adapted and non-adapted powdery mildew fungi

Clone ID a RNAi target gene Description (Blast X) b Susceptible cells (%)
Mean ± SE P c α (FDR 0.1) d
  Positive ctre   4.84 ± 0.57 0.0001 0.0023
  Negative ctrf   0.10 ± 0.04 ---  
HM01A17 Rnr1 Nonclathrin coat protein γ 1 1.08 ± 0.33 0.007 0.0070
HO10B14 Rnr2 BAH domain protein 0.35 ± 0.17 0.029 0.0140
HO14B18 Rnr4 Endo-1.4-beta-glucanase 0.56 ± 0.18 0.005 0.0047
HO14H18 Rnr5 ARM Repeat protein 0.35 ± 0.19 0.007 0.0093
HO02M14 Rnr6 Cellulose-synthase like D2 1.29 ± 0.49 0.036 0.0163
HO15P19 Rnr7 EF Hand protein 1.15 ± 0.43 0.048 0.0256
HO27O23 Rnr8 Receptor-like kinase 0.74 ± 0.32 0.044 0.0233
HU02G09 Rnr9 Subtilisin-like protein 0.50 ± 0.23 0.009 0.0186
HW03O11 Rnr10 Stomatin-like protein 0.54 ± 0.30 0.044 0.0116
  1. aEST clone ID deposited at NCBI that was used for generation of the TIGS construct.
  2. bBlast X results with an E-value of lower than 10-10.
  3. cMann-Whitney test against empty-vector control (one-tailed). P values lower/equal than Benjamini-Hochberg-corrected significance threshold α (FDR 0.1) are highlighted in italics.
  4. dSignificance threshold with false-discovery rate (FDR) 0.1.
  5. eSilencing of Hv-SNAP34 (Rnr3; Douchkov et al. [23]).
  6. fEmpty-vector control pIPKTA30N.