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Table 3 Identification of Rnr genes required for NHR of barley to B. graminis f.sp. tritici

From: Discovery of genes affecting resistance of barley to adapted and non-adapted powdery mildew fungi

Clone ID a

RNAi target gene

Description (Blast X) b

Susceptible cells (%)

Mean ± SE

P c

α (FDR 0.1) d

 

Positive ctre

 

4.84 ± 0.57

0.0001

0.0023

 

Negative ctrf

 

0.10 ± 0.04

---

 

HM01A17

Rnr1

Nonclathrin coat protein γ 1

1.08 ± 0.33

0.007

0.0070

HO10B14

Rnr2

BAH domain protein

0.35 ± 0.17

0.029

0.0140

HO14B18

Rnr4

Endo-1.4-beta-glucanase

0.56 ± 0.18

0.005

0.0047

HO14H18

Rnr5

ARM Repeat protein

0.35 ± 0.19

0.007

0.0093

HO02M14

Rnr6

Cellulose-synthase like D2

1.29 ± 0.49

0.036

0.0163

HO15P19

Rnr7

EF Hand protein

1.15 ± 0.43

0.048

0.0256

HO27O23

Rnr8

Receptor-like kinase

0.74 ± 0.32

0.044

0.0233

HU02G09

Rnr9

Subtilisin-like protein

0.50 ± 0.23

0.009

0.0186

HW03O11

Rnr10

Stomatin-like protein

0.54 ± 0.30

0.044

0.0116

  1. aEST clone ID deposited at NCBI that was used for generation of the TIGS construct.
  2. bBlast X results with an E-value of lower than 10-10.
  3. cMann-Whitney test against empty-vector control (one-tailed). P values lower/equal than Benjamini-Hochberg-corrected significance threshold α (FDR 0.1) are highlighted in italics.
  4. dSignificance threshold with false-discovery rate (FDR) 0.1.
  5. eSilencing of Hv-SNAP34 (Rnr3; Douchkov et al. [23]).
  6. fEmpty-vector control pIPKTA30N.