Discovery of genes affecting resistance of barley to adapted and non-adapted powdery mildew fungi

Table 2 Comparison of TIGS effects between candidate-gene groups for quantitative host resistance (QR)

Selection criterion	Family	Entry ^a	Repeated ^b	Significant TIGS effect ^c
		Genes (n)	Genes (n)	Genes (n)	%	P ^d
Regulated	Multiple	345	112	34	9.9	0.084
Random	Multiple	87	33	4	4.6	-
Gene family	ABC transporter	90	19	8	8.9	0.372
	Cellulose-synthase like	22	15	5	22.7	0.016
	LysM-, LRR-RLK	35	10	7	20.0	0.013
	Class III Peroxidase	69	16	6	8.7	0.339
	Proteasome lid comp.	51	17	3	5.9	0.709
	U box-, RING-E3 ligase	36	7	4	11.1	0.231
	Sweet sugar transporter	12	12	4	33.3	0.007
	WKRY TF	58	16	7	12.1	0.116
QTL ci^e	Multiple	109	31	8	7.3	0.554
Total		914	288	90	9.8	-

^aNumber of TIGS target genes for first screening round.
^bRelative to the number of genes targeted by repeated bombardments of TIGS constructs.
^cSignificant deviation from median value of all bombarded TIGS constructs (1-sample t-test, α 0.05, one-tailed).
^dTest for significant over-representation of specific gene groups associated with a TIGS effect, compared to the set of randomly selected genes (Fisher’s exact, one-tailed).
^eConfidence interval for resistance QTL on chromosome 5H (peak-marker LOD -1).

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