TIGS of host genes affects the interaction of barley with the powdery mildew fungus Bgh . (A) Distribution of log2-transformed relative SI values of all tested TIGS constructs in the first screening round. (B) Ordered mean values of TIGS effects of all constructs bombarded in at least five independent experiments, after the selection based on results from the first screening round. For completeness this overview includes 13 target genes to be reported later elsewhere. Green and red bars, significantly enhanced susceptibility and resistance, respectively (one-tailed P <0.05). (C) Ordered mean values of significant TIGS effects (P <0.05, one-tailed one-sample t-test) of all constructs bombarded in at least five independent experiments. The results are grouped according to a manual, broad functional-category assignment of corresponding target genes. CW, cell wall; expr., expression; metab., metabolism; Prim., primary; sec., secondary; secr., secretion; protein, protein translation, modification, or degradation; *, Polyubiquitin genes.