Induction of halofuginone resistance occurs in eight generations and is stable. After eight generations of exposure to 4× EC50 of halofuginone (2.8 nM), both Dd2 replicates, Dd2 Induced 1 and 2, recrudesced and became 20- to 30-fold resistant to halofuginone treatment. This phenotype was stable during freeze-thaws and after 30+ generations of growth of Dd2 Induced 1 and 2 in the absence of halofuginone (Dd2-Induced 1 Off Pressure and Dd2-Induced 2 Off Pressure). EC50s were determined by SYBR green growth assay. Error bars denote standard deviation.