K-mer Coverage across the Kasalath/ Pstol1 gene in the three genomes, with 30 kbp of upstream and downstream flanking sequence. The k-mer coverage is plotted with respect to the reference Kasalath sequence (AB458444.1). The position of the Pstol1 gene is indicated with green vertical bars. Also see Figure S9 in Additional file 1 for a detailed view of the Pstol1 coverage, and Figure S10 in Additional file 1 for a plot of the entire Kasalath sequence. Unresolved gaps in the reference sequence are indicated with black vertical bars. Only DJ123 has consistent coverage across this region, especially upstream of the gene, while the other two genomes show complete gaps in coverage.