Phylogenetic tree of six Legionella species and seven L. pneumophila strains and their shared Dot/Icm substrates. Neighbor-joining tree based on the concatenation of 816 protein-coding genes from 11 Legionella genomes. C. burnetii was used as out-group. The tree was constructed using MEGA and JTT as model of evolution. The values above nodes indicate the bootstrap values. The values in blue circles represent the number of Dot/Icm substrates shared by the species in the corresponding cluster, suggesting that they were present in the common ancestor. The values inside blues squares are the number of Dot/Icm substrates shared between L. pneumophila strains and the remaining species (for example, the species L. micdadei and L. pneumophila share 33 Dot/Icm substrates).