Intracellular replication of L. hackeliae , L. micdadei and L. fallonii (LLAP10). (A) THP-1 derived macrophages at 37°C. (B) A. castellanii culture at 20°C. (C) A. castellanii plate test at 37°C and 30°C. L. pneumophila strain Paris wild type (wt) and ΔdotA were used as positive and negative controls, respectively. Intracellular replication for each strain was determined by recording the number of colony-forming units (CFU) through plating on BCYE agar. Blue, L. pneumophila strain Paris; red, ΔdotA; orange, L. micdadei; violet, L. hackeliae; green, L. fallonii (LLAP10). Results are expressed as Log10 ratio CFU Tn/T0 and each point represents the mean ± standard deviation of two or three independent experiments. The error bars represent the standard deviation, but some were too small to clearly appear in the figure.