Figure 2

ST191 outbreak isolates are represented by multiple genetic subtypes which arose by mutation, recombination and horizontal gene transfer. (a) Genome distribution of mutations and predicted recombinant regions among ST191 outbreak isolates. Polymorphisms are mapped to the reference strain Corby. SNPs are represented by short black lines, and variant T4SSs are indicated by colored circles; Dot/Icm T4BSS (variant 1), dark green; Dot/Icm T4BSS (variant 2), light green; Lvh T4ASS (Philadelphia), red; Lvh T4ASS (novel), blue. Predicted recombinant regions are flanked by dashed red lines. (b) Maximum likelihood un-rooted radial phylogeny reconstructed using the non-recombinant core genome of ST191 outbreak isolates. For each node, maximum likelihood bootstrap values are displayed. Filled triangles indicate multiple isolates that were obtained from a single patient as follows; patient 1, yellow; patient 2, red; patient 12, green; patient 13, blue. Presence or absence of genomic regions encoding T4SS is indicated by colored filled circles as per (a).