Figure 8From: Transcriptomic analysis reveals tomato genes whose expression is induced specifically during effector-triggered immunity and identifies the Epk1 protein kinase which is required for the host response to three bacterial effector proteinsSlEpk1 appears to act upstream of MAPK signaling. Leaves of N. benthamiana plants silenced for Ec1, SlEpk1, MAPKKKα, MEK2 or SlPrf were syringe-infiltrated with Agrobacterium carrying transgenes for MAPKKKα, MAPKKKα KD-, MEK2DD or MEK2 WT. PCD percentage was calculated as described in the Materials and methods using six plants per construct. Asterisks indicate significant differences compared with Ec1-silenced plants using Fisher’s exact test (P <0.05). PCD, programmed cell death; MEK2DD, constitutive-active MAP kinase kinase 2; MEK2 WT, wild type MEK2; MAPKKKα KD-, MAPKKKα with an inactive kinase domain.Back to article page