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Figure 6 | Genome Biology

Figure 6

From: A systematic genetic screen identifies new factors influencing centromeric heterochromatin integrity in fission yeast

Figure 6

Csn1 and Csn2 contribute to heterochromatin integrity via regulation of Epe1. (A, B) Analysis of genetic interactions of csn1? and csn2? with ddb1?. (A) Assays for silencing of reporter genes at the centromere (cen1:ade6+) and mating-type locus (mat3-M:ura4+). Plates are limiting adenine (LOW ADE), non-selective (N/S), lacking uracil (-URA) or supplemented with FOA (+FOA). (B) qRT-PCR analysis of centromeric outer repeat (cen-dg) and mating-type locus (mat) transcript levels relative to act1+, normalised to wild-type. (C) ChIP analysis of Epe1-FLAG levels at centromeric outer repeats (cen-dg) relative to act1+, normalised to wild-type. (D) Assay for silencing at the mating-type locus (mat3-M:ura4+).

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Genome Biology

ISSN: 1474-760X

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