Sde2 and Saf5 are involved in splicing. (A) Synthetic interaction with cwf11?. Equivalent cell numbers of the indicated strains were spotted in serial dilutions and incubated at 32°C for 3 days, or 25°C for 4 days. (B) Splicing assay. Accumulation of intron relative to exon RNA in wild-type versus mutant strains was measured for nda2+ and nda3+ by qRT-PCR analysis using primer pairs either spanning an intron-exon boundary or within an exon, as illustrated above. (C) Splicing of centromeric outer repeat (cen-dg) non-coding transcripts. Accumulation of spliced and unspliced cen(dg) transcripts were monitored by RT-PCR using primers flanking a previously reported intron sequence.