Figure 4From: A systematic genetic screen identifies new factors influencing centromeric heterochromatin integrity in fission yeastSde2 and Saf5 are involved in splicing. (A) Synthetic interaction with cwf11?. Equivalent cell numbers of the indicated strains were spotted in serial dilutions and incubated at 32°C for 3 days, or 25°C for 4 days. (B) Splicing assay. Accumulation of intron relative to exon RNA in wild-type versus mutant strains was measured for nda2+ and nda3+ by qRT-PCR analysis using primer pairs either spanning an intron-exon boundary or within an exon, as illustrated above. (C) Splicing of centromeric outer repeat (cen-dg) non-coding transcripts. Accumulation of spliced and unspliced cen(dg) transcripts were monitored by RT-PCR using primers flanking a previously reported intron sequence.Back to article page