Sde2, Smd3, Saf1 and Saf5 affect RNAi-dependent heterochromatin formation. (A) cen1:ade6+ silencing assay. Equivalent cell numbers of the indicated strains were spotted in serial dilutions on non-selective plates (N/S), or plates containing 10 ug/mL adenine (LOW ADE) or no adenine (-ADE). (B) ChIP analysis of H3K9me2 levels at the centromeric outer repeats (cen-dg) relative to the act1+ gene, normalised to wild-type. (C) Northern analysis of centromeric siRNAs. snoRNA58 (snR58) is a loading control.