Figure 2

The majority of screen hits cause de-repression at centromeric outer repeats. (A, B) qRT-PCR analysis of centromeric outer repeat (A; cen-dg) and mating-type locus (B; mat) transcript levels relative to act1⁺, normalised to wild-type. Along with clr4? and dcr1?, sir2? was included as a control for the sensitivity of the assay since this mutant is known to cause only mild de-repression at the centromeric locus analysed. (C) ChIP analysis of RNAPII levels at the centromeric outer repeats (cen-dg) relative to tRNA in lsk1? mutant cells. (D) ChIP analysis of H3K9me2 levels at the centromeric outer repeats (cen-dg) and cen1:ade6⁺ reporter gene relative to the act1⁺ gene in lsk1? mutant cells.