Figure 1From: A systematic genetic screen identifies new factors influencing centromeric heterochromatin integrity in fission yeastGenetic screen for mutants defective in heterochromatic silencing. (A) Schematic representation of the screening strategy. High-throughput crossing of a gene deletion library to a tester strain (see text for further details) facilitated analysis of the effects of individual gene deletions on silencing of an ade6+ reporter gene inserted into the centromeric outer repeats of centromere 1 (cen1:ade6+; otr (dg and dh): outer repeats; imr: innermost repeats; cnt: central core) (B) Illustration of the semi-quantitative system used to score defects in cen1:ade6+ silencing, based on colony colour on plates containing limiting levels of adenine (LOW ADE) or colony size on plates lacking adenine (-ADE), where 4 is equivalent to wild-type silencing, and 1 indicates strong ade6+ de-repression.Back to article page