Loss of DNA methylation uncovers thousands of distal H3K27ac sites. (A) Venn diagram comparing distal H3K27ac peaks in HCT116 and DKO1 cells. (B) DNA methylation status of common and unique enhancers. Pie charts show the breakdown of genomic locations for peaks residing within intergenic, intronic, and exonic regions of the genome. Tag density plots show the density of H3K27ac tags relative to the centers of these peaks. (C) Distances from the TSS of genes up-regulated (right panel) or down-regulated (left panel) in DKO1 to the nearest categorized enhancer. Also indicated is the median distance from each category of enhancer to the nearest up-regulated or down-regulated gene.