Whole genome bisulfite sequencing comparative analysis of HCT116 and DKO1 cells. (A) Light blue and pink tracks represent the sequencing coverage along a segment of human chromosome 19 in HCT116 and DKO1 cells, respectively. Dark blue and red tracks illustrate the percentage of methyl-C/C in HCT116 and DKO1, respectively. Light-colored lines within the percentage methylation (%mCpG) tracks represent the average percentage methylation in the immediate region. CpG islands are shown above the RefSeq genes as green bars. (B) Box plot illustrating the percentage methylation in promoters, gene bodies, and random regions of the genome; the horizontal line in each bar indicates the median value. For HCT116 cells the median values are 30% (promoters), 84% (gene bodies), and 84% (random regions) and for DKO1 cells the median values are <1% (promoters), 13% (gene bodies), and 9% (random regions). (C) The number of promoters containing varying levels of methylation in HCT116 and DKO1 are shown; the minimum and maximum DNA methylation values for the region between -100 and +700 relative to the start site at the promoters in each group is indicated by the color key.