Gene clustering according to expression profile. Twenty groups of genes were clustered by expression profile. The expression profiles used for grouping were generated using 11 RNA-seq samples spanning developmental time points including: 0 to 1, 2 to 4, 4 to 8, and 8 to 12 h embryos, larva, pupa, adult males, adult females, non-blood-fed ovaries, blood-fed ovaries, and 24 h post-blood-fed female carcass without ovaries. Male stage are colored blue, female stages are colored green, ovary samples are colored yellow, embryo samples are colored red, larva samples are colored pink, and pupa samples are colored purple. Many of these clusters correspond to either a specific developmental stage or specific sex.