Figure 5

Distribution of polymorphisms in miRNA genes and surrounding regions from 26 ecotypes of M. truncatula. Box plots of the number of SNPs (expressed as SNP/100 bp) at M. truncatula miRNA loci, as a function of their location with respect to the pre-miRNA. Data for conserved and novel miRNAs are displayed. Upstream-1000 corresponds to the upstream region from -1,500 to -1,000; upstream-500 corresponds to the upstream region from -999 to -500 and upstream-1 corresponds to the upstream region from -499 to -1. The same nomenclature applies for downstream regions. Precursor stands for pre-miRNA stem-loops and mature stands for 21 or 22 nucleotide miRNA. The blue point indicates the median of SNP abundances. The grey boxes contain 50% of the data and notches indicate the median confidence interval. Whiskers indicate the upper and lower quartiles and no whisker is visible if the lower quartile is equal to the minimum (in that case, the grey boxe contains 75% of the data). Outliers (more than 1.5 interquartile range) are depicted by open circles.