Table 1 Statistical analysis of sequencing reads from in silico simulation and RRHP experiment
From: RRHP: a tag-based approach for 5-hydroxymethylcytosine mapping at single-site resolution
| Â | Simulation | RRHP-HpaII | RRHP-MspI-1 | RRHP-MspI-2 | RRHP-MspI-3 | RRHP | RRBS |
|---|---|---|---|---|---|---|---|
40-430 bp | (0.5 μg) | (0.5 μg) | (0.5 μg) | (0.1 μg) | (- βgt control) | ||
Total reads | 1,845,334 | 23,702,341 | 23,383,403 | 9,505,230 | 19,539,657 | 5,518 | 41,066,513 |
Mapped reads | 1,845,025 | 20,605,538 | 22,271,499 | 9,017,016 | 18,482,119 | 4,373 | 15,668,523 |
Mappability (%) | 99.98 | 86.93 | 95.24 | 94.86 | 94.59 | 79.25 | 38.15 |
Tagged reads (n) | 1,845,025 | 17,763,501 | 21,081,749 | 8,531,903 | 17,328,911 | 3,230 | NA |
Tagged reads (%) | 100 | 86 | 95 | 95 | 94 | 74 | NA |
5hmc sites (n) | 1,845,014 | 1,878,394 | 1,737,993 | 1,550,791 | 1,674,080 | 3,171 | 5,330,488 |