Figure 4
From: RRHP: a tag-based approach for 5-hydroxymethylcytosine mapping at single-site resolution
5hmC is asymmetrically distributed between the forward and reverse strand throughout the genome. (a) Distribution of the read count difference (log2 scale) between the forward and reverse strand for sample RRHP-MspI-1. (b) CpG sites with asymmetric 5hmC distribution between the forward and reverse strand were analyzed according to gene annotation (column plot) and coding (pie chart). Legend for annotation column plot - Green: detectable MspI sites; Yellow: MspI site with 5hmC detected from both strands; Red: MspI sites with two-fold 5hmC difference between the strands. Legend for pie chart - Green: coding strand; Yellow: non-coding strand.