Our re-analysis of published primary/relapse AML dataset in Ding et al. Primary, relapse, and merged subclone structures for two patients, reconstructed with SubcloneSeeker. (A) SubcloneSeeker analysis found six alternative primary subclone structures for patient UPN933124. Only one is compatible with the relapse subclone structure, and the pair is in agreement with the original study. (B) Each of the two viable merged primary/relapse subclone structures for patient UPN75816 suggests that the two primary subclones made it to the relapse tumor, and further expanded.