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Table 2 Optimal properties of images for analysis in GoIFISH GoIFISH

From: GoIFISH: a system for the quantification of single cell heterogeneity from IFISH images

 

GUI

GoIFISHWrapper (Command Line)

File Format

.mat or.tiff

.tiff.zvi Use bio-formats to convert other formats

Image Size

12 megapixels for comfortable use

Theoretically unlimited

Number of stains

Up to 5. Must include DAPI

Unlimited but must include DAPI

Number of Cells

Under 1000 for comfortable use

Theoretically unlimited

Cell Size (px)

Optimally 60x magnification (2500-10000 px), 20x and 40x magnification

also available (250-3000 px)