From: GoIFISH: a system for the quantification of single cell heterogeneity from IFISH images
GUI | GoIFISHWrapper (Command Line) | |
---|---|---|
File Format | .mat or.tiff | .tiff.zvi Use bio-formats to convert other formats |
Image Size | 12 megapixels for comfortable use | Theoretically unlimited |
Number of stains | Up to 5. Must include DAPI | Unlimited but must include DAPI |
Number of Cells | Under 1000 for comfortable use | Theoretically unlimited |
Cell Size (px) | Optimally 60x magnification (2500-10000 px), 20x and 40x magnification | also available (250-3000 px) |