Figure 3
From: GoIFISH: a system for the quantification of single cell heterogeneity from IFISH images
Correlation between measured intensities and classification by a pathologist. Comparison of semi-quantitative scoring by a pathologist with CellProfiler, Columbus and GoIFISHGoIFISH quantitation in (A) ER staining, (C) HER2 membrane completeness using mean intensities (E) HER2 membrane intensity using coefficient of variance. Combined distributions of quantitations across all 10 samples for (B) ER positivity, (D) HER2 membrane completeness using mean intensities and (F) HER2 membrane intensity using coefficient of variance. Note that GoIFISHGoIFISH nuclear adjusted intensities were used in (A, B), GoIFISHGoIFISH background adjusted intensities were used in (C, D) and GoIFISHGoIFISH raw intensities used in (E, F).