Figure 4

Location, strength of occupancy, and inferred activity of GBRs compared pairwise between GRα and mutants. (A) Example of allele-specific GBR location: HOXD1 gene displays proximal GRα-specific and A477T-specific GBRs. (B-D) Strength of occupancy: Pie charts of relative magnitude of GBR occupancy (P <0.05) in pairwise comparisons of GRα relative to (B) A477T, (C) 30iiB, and (D) E773R: greater mutant occupancy (blue), greater GRα occupancy (yellow) or not significantly different occupancy (grey). There was enrichment of GRα-selectively occupied GBRs near genes that lost activation (E) in cells expressing A477T. Similarly, there was enrichment of A477T-selectively occupied GBRs near genes that gained activation (F), although such genes also displayed enrichment for GRα-selectively occupied GBRs. The relative heights of the enrichment curves (E and F) are proportional to the number of binding sites of each allele. In contrast, 30iiB and E773R GBRs were generally similar to GRα GBRs in both location and strength of occupancy.