Discovery of novel transcripts and gametophytic functions via RNA-seq analysis of maize gametophytic transcriptomes

Table 7 Transmission frequency of Ds insertions in genes with high and low gametophyte expression

	Dsinsertions with reduced transmission
Female transmission of Ds insertions in genes with highest expression in the Embryo Sac	2/8
Male transmission of Ds insertions in genes with highest expression in the Pollen	2/9
Transmission through the opposite gametophyte for genes with highest expression in the Embryo Sac or Pollen	0/16
Transmission through either gametophyte for genes with highest expression in one of the sporophyte tissues	0/16 (or 1/16*)

Reciprocal crosses were made between wild-type W22 plants and plants carrying Ds insertions in genes with varying expression patterns. Ds insertions that were recovered in fewer than 50% of the progeny (p < 0.05) were scored as having reduced transmission. See Additional file 21: Table S13 for supporting data.
*(One of these Ds lines is on the borderline of being significantly lower than 50% (p = 0.0474).
The frequency of Ds insertions with reduced transmission is significantly higher for genes with the highest expression in the gametophyte tested (4/17) than other genes (Fisher’s Exact Test p = 0.011, for 0/32 non-gametophyte genes, and Fisher’s Exact Test, p = 0.043, for 1/32 non-gametophyte genes).

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