Phylogeny and expression levels of putative T. melanosporum DMTs. (A) Maximum likelihood tree based on the catalytic domains of DNMT1 and DNMT1-related DMTs. Bacterial DMTs were used as outgroup; divergent enzymes belonging to the DNMT3 group were not considered. (B) Maximum likelihood analysis conducted as in (A) on RID-like proteins from various fungi. Source organism name and accession numbers are indicated for each sequence. Bootstrap values (1,000 replicates) are indicated for each branch. Additional DNMT1 domains, besides the catalytic domain (DCM), are indicated on the right side near to ‘reference’ fungal proteins (BAH, bromo-adjacent homology; RFD, replication foci domain). (C) Expression levels (RPKM) of the RID/Masc1-like (TmelDNMT1, GSTUMT00012206001; left bars) and the DIM-2-like (TmelDNMT2, GSTUMT00003328001; right bars) Tuber DMTs in FB (blue bars) and FLM (red bars).