Figure 7

Genes upregulated by AzaC in AML3 are preferentially impacted by mutations that affect DNA methylation and upregulated by Decitabine in patient primary AML blasts. (a) Difference between mean promoter methylation of normal karyotype primary AML WT or mutant at DNMT3A R882 for the indicated groups of genes (all, AML3_AzaC_WIMSi_246 (Aza/246) or three groups of 246 randomly selected genes). P value (Wilcoxon test) of AML3_AzaC_WIMSi_246 versus all other groups <0.001. (b) As (a), but the difference between IDH1 R132 and WT. P value of AML3_AzaC_WIMSi_246 versus all other groups <0.001. (c) As (a), but the difference between IDH2 R140 and WT. P value of AML3_AzaC_WIMSi_246 versus all other groups <0.001. (d) Log2 fold change in expression of AML3_AzaC_WIMSi_246 genes (blue) and all genes (red) in patient primary blasts after treatment with decitabine. Data from [9]. Sixteen out of 17 patients showed a P value <0.001 (Fisher’s Exact). See Additional file 5: Dataset 10 for P value of difference between AML3_AzaC WIMSi (246) genes and all genes. (e) As (a), but 662 genes out of 1,147 genes regulated by AzaC in AML3 (Additional file 5: Dataset 1) that are most divergent from signature identified by WIMSi depicted in Figure 6c (AML3_AzaC_662). See Additional file 5: Dataset 10 for P value of difference between AML3_AzaC WIMSi (246) genes and AML3_AzaC_662 genes.