Table 1 Yeast strains used in this study
Name | Background | Genotype | References |
|---|---|---|---|
Y239 | S288C | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 | |
Y240 | S288C | MATalpha his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 | |
YK32 | S288C | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 NOP1-FLAG::KANMX | This study |
YK68 | S288C | MATalpha his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 SNU13-FLAG::KANMX | This study |
YK3 | S288C | MATa leu2 his3 ura3 lys2 NOP56-FLAG::KANMX | [14] |
YK23 | S288C | MATalpha his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 NOP58-FLAG::KANMX | This study |
YK28 | S288C | MATalpha his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 NOP58-FLAG::KANMX pih1Δ::URA3 | This study |
YK29 | S288C | MATalpha his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 NOP58-FLAG::KAN tah1Δ::URA3 | This study |
YK26 | S288C | MATalpha his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 RVB1-FLAG::KANMX | This study |
YK27 | S288C | MATalpha his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 RVB2-FLAG::KANMX | This study |
YK31 | S288C | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 PIH1-FLAG::KANMX | This study |
rvb1-DAmP | S288C | MATalpha can1Δ ::STE2pr-Sp_his5 lyp1Δ ::STE3pr-LEU2 RVB1-3'UTR::NAT | [36] |
rvb2-DAmP | S288C | MATalpha can1Δ ::STE2pr-Sp_his5 lyp1Δ ::STE3pr-LEU2 RVB2-3'UTR::NAT | [36] |
R0055 | S288C | MATalpha can1Δ∷MFA1pr-HIS3 his3Δ1 leu2Δ0 ura3Δ0 met15Δ0 lys2Δ0 pih1Δ∷NAT | [21] |
R0061 | S288C | MATalpha can1Δ∷STE2pr-Sp_his5 lyp1Δ his3Δ1 leu2Δ0 ura3Δ0 met15Δ0 tah1Δ∷URA3 | [14] |
nop58-3′Δ | S288C | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 yor309cΔ∷KANMX | Open Biosystems |
RVB1-GFP | S288C | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 RVB1-GFP::HIS3 | Life Technologies |
YK169 | S288C | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 RVB2-GFP::KANMX | This study |
PIH1-GFP | S288C | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 PIH1-GFP::HIS3 | Life Technologies |
TAH1-GFP | S288C | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 TAH1-GFP::HIS3 | Life Technologies |
NOP1-GFP | S288C | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 NOP1-GFP::KANMX | This study |
NOP56-GFP | S288C | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 NOP56-GFP::HIS3 | Life Technologies |
NOP58-GFP | S288C | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 NOP58-GFP::HIS3 | Life Technologies |
RVB1-GFP pih1Δ | S288C | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 RVB1-GFP::HIS3 pih1Δ::NAT | This study |
TAH1-GFP pih1Δ | S288C | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 TAH1-GFP::HIS3 pih1Δ::NAT | This study |
NOP1-GFP pih1Δ | S288C | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 NOP1-GFP::KANMX pih1Δ::NAT | This study |
NOP56-GFP pih1Δ | S288C | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 NOP56-GFP::HIS3 pih1Δ::NAT | This study |
NOP58-GFP pih1Δ | S288C | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 NOP58-GFP::HIS3 pih1Δ::NAT | This study |
kap121-34 PIH1-GFP | DF5 | MATa ura3-52 his3-Δ200 trp1-1 leu2-3,112 lys2-801 kap121Δ::LEU2 pkap121-34-TRP1 PIH1-GFP::HIS3 | This study |
kap121-34 TAH1-GFP | DF5 | MATa ura3-52 his3-Δ200 trp1-1 leu2-3,112 lys2-801 kap121Δ::LEU2 pkap121-34-TRP1 TAH1-GFP::HIS3 | This study |
YK124 | S288C | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 PIH1-GFP::HIS3 [pYEX-NUP53] | This study |
YK125 | S288C | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 [pYEX-NUP53 Δ405-430] | This study |
YK175 | S288C | MATa his3Δ1 leu2Δ0 ura3Δ0 crm1-T539C PIH1-GFP::HIS3 | This study |
YK176 | S288C | MATa his3Δ1 leu2Δ0 ura3Δ0 met15Δ0 crm1-T539C TAH1-GFP::HIS3 | This study |