Figure 7
R2TP nuclear import and export is dependent on Kap121 and Crm1. (A) Subcellular localizations of endogenously GFP-tagged Pih1 and Tah1 in wildtype background and in kap121-34 mutant cells at the permissive (26°C) and restrictive (30°C) temperatures in log phase cells. (B) Subcellular localizations of endogenously GFP-tagged Pih1 and Tah1 in WT cells expressing Nup53-CΔ or Nup53 under CUP1 promoter in log phase cells. (C) Western blot analysis of R2TP complex purified from log phase cells expressing Nup53-CΔ or Nup53. (D) Subcellular localizations of endogenously GFP-tagged Pih1 and Tah1 in crm1-T539C background strain in the absence or presence of LMB in stationary phase.