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Figure 3 | Genome Biology

Figure 3

From: Nutritional status modulates box C/D snoRNP biogenesis by regulated subcellular relocalization of the R2TP complex

Figure 3

Analysis of R2TP complex interaction with mature and premature box C/D snoRNA. (A) Northern blot analysis of mature U14 snoRNA in pulldown complexes of Pih1-FLAG (R2TP) and Nop56-FLAG (box C/D snoRNP); 6 μg and 0.6 μg of total RNA and RNAs purified from the pulldown complexes are shown. WT (no tag) strain was used as a negative control. (B) RT-PCR analysis of premature U14 snoRNA in pulldown complexes of R2TP (Rvb1-, Rvb2-, and Pih1-FLAG) and box C/D snoRNP (Nop1-, Snu13-, Nop56-, and Nop58-FLAG). RNAs were purified from the pulldown complexes and PCR was performed with (+RT) or without (−RT) reverse transcription.

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