Figure 2

Effect of R2TP complex on Nop58 stability. (A) Western blot analysis of steady-state protein levels of box C/D snoRNP protein components in WT, rvb1-DAmP, rvb2-DAmP, pih1Δ, and tah1Δ strains in log phase cells. (B) Western blot analysis of protein levels of box C/D snoRNP core proteins in NOP56-FLAG pih1Δ and SNU13-FLAG pih1Δ strains transformed with p416GAL-PIH1 plasmid. Cells were shifted from galactose- to glucose-containing medium, and protein levels were analyzed as a function of time. (C) Western blot analysis of Nop58 and Pih1 steady-state levels in log phase WT and nop58-3′Δ cells.