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Figure 1 | Genome Biology

Figure 1

From: Timber! Felling the loblolly pine genome

Figure 1

Major innovations in sequencing and assembly, coupled with reductions in genome complexity, have led to major milestones in sequencing plant genomes, as highlighted by the loblolly pine genome. Highlighted in the blue arrow are selected plants in which technical innovations in sequencing technology, genome assembly and/or a reduction in genome complexity were instrumental in achieving an assembled genome. Shown on the left (in blue) are major innovations in overall sequencing technologies, approaches and assembly starting with pre-2000 through to 2014 (OLC, overlap layout consensus; BAC, bacterial artificial chromosome; WGS, whole-genome shotgun sequencing; 2nd gen sequencing, second-generation sequencing; DBG, de Bruijn graph; 3rd gen L-R sequencing, third-generation long-read sequencing; 3rd gen L-R assembly, third-generation long-read assembly). Shown on the right (in black) are selected plants for which a milestone was achieved: Arabidopsis thaliana (119 Mbp assembly, 2000), Oryza sativa (rice, 390 Mbp assembly, 2002), Vitis vinifera (grapevine, 487.1 Mbp assembly, 2007), Cucumis sativus (cucumber, 243.5 Mbp assembly, 2009) Fragaria vesca (strawberry, 220 Mbp assembly, 2011), Solanum tuberosum (potato, 727 Mbp assembly, 2011), and Pinus taeda (loblolly pine, 22 Gbp assembly, 2014).

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