Figure 6

mpk3 shows enhanced flg22-mediated activation of SA-mediated defences. SA accumulation 24 h after mock-treatment or treatment with 1 μM flg22 in Col-0 and mpk4 (A) and in Col-0, mpk3 and mpk6 seedlings (B). Bars are means ± SD. (C) qPCR analysis of the expression of SA marker genes SID2, EDS1 and PAD4 and the ET/JA marker gene PDF1.2 in Col-0 and mpk3 seedlings 24 h after treatment with 1 μM flg22. Transcript accumulation is expressed relative to the reference gene ACTIN2. Bars represent means ± SE of three independent biological replicates and each replicate is composed of three technical replicates. Stars indicate significant difference with Col-0 under the same conditions based on a two-tailed Student’s ttest. **P value <0.01 and *P <0.05.