IVT-seq reveals extreme bias in RNA sequencing

Table 1 Detection of source cDNA sequences in IVT-seq

Total number of cDNA clones:	963
	Replicate 1	Replicate 2
Number of clones detected (FPKM ≥5):
IVT only	869	870
1:1 Mix	877	876
1:2 Mix	886	883
1:10 Mix	896	892
Mouse only	278	271
PolyA selection	829	-
No selection	870	-
Plasmid library	924	-
Average, normalized^a depth of coverage for detected clones:
IVT Only	76.09	80.22
1:1 Mix	75.15	75.06
1:2 Mix	65.79	69.40
1:10 Mix	37.50	47.46
Mouse only	01.58	02.42
PolyA selection	72.27	-
No selection	72.74	-
Plasmid library	42.08	-

^aAverage depth of coverage is normalized by the number of millions of fragments mapped to the human reference in each sample.

ISSN: 1474-760X