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Table 1 Detection of source cDNA sequences in IVT-seq

From: IVT-seq reveals extreme bias in RNA sequencing

Total number of cDNA clones: 963
  Replicate 1 Replicate 2
Number of clones detected (FPKM ≥5):   
IVT only 869 870
1:1 Mix 877 876
1:2 Mix 886 883
1:10 Mix 896 892
Mouse only 278 271
PolyA selection 829 -
No selection 870 -
Plasmid library 924 -
Average, normalizeda depth of coverage for detected clones:   
IVT Only 76.09 80.22
1:1 Mix 75.15 75.06
1:2 Mix 65.79 69.40
1:10 Mix 37.50 47.46
Mouse only 01.58 02.42
PolyA selection 72.27 -
No selection 72.74 -
Plasmid library 42.08 -
  1. aAverage depth of coverage is normalized by the number of millions of fragments mapped to the human reference in each sample.