Conserved microRNA editing in mammalian evolution, development and disease

Table 1 Detected miRNA editing events that were shared between at least two species

ID	Pos.	Human	Macaque	Mouse	Opossum	Platypus	Chicken	Human SNP	Opossum SNP	Known	Validated
miR-27a	6	>1%	>1%			>1%		No	-	Human^e	-
miR-27a	6	>1%	>1%			>1%		No	-	-	-
miR-99b*	2		>1%	>1%				No	-	Human^c	-
miR-99b*	2		>1%	>1%				No	-	Mouse^c	Mouse^c
miR-140*	16				>5%		>5%	No	No	-	-
miR-140*	16				>5%		>5%	No	No	-	-
miR-187*	5			>1%	>1%			No	No	-	-
miR-187*	5			>1%	>1%			No	No	-	-
miR-301a	20		>1%		>5%		>5%	No	No	-	-
miR-301a	20		>1%		>5%		>5%	No	No	-	-
miR-376a-1	3	>1%	>1%	>1%				No	-	Human^b,c,d	-
miR-376a-1	3	>1%	>1%	>1%				No	-	Mouse^b,c,d,e,f	Mouse^b,c
miR-376b	6	>5%		>5%				No	-	Human^b,c,d	-
miR-376b	6	>5%		>5%				No	-	Mouse^b,c,d,f,g	Mouse^b,c
miR-376c	6	>5%		>5%				No	-	Human^e	-
miR-376c	6	>5%		>5%				No	-	Mouse^b,d,e,f	Mouse^b
miR-379	5		>5%	>5%				No	-	Human^a,c,e	-
miR-379	5		>5%	>5%				No	-	Mouse^c,d,e,f	Mouse^c
miR-381	4	>5%	>5%	>5%				No	-	Human^e	Human^e
miR-381	4	>5%	>5%	>5%				No	-	Mouse^d,f,g	-
miR-411	5	>5%	>5%	>5%				No	-	Human^b,d	-
miR-411	5	>5%	>5%	>5%				No	-	Mouse^c,d,e	Mouse^c
miR-455	17		>1%				>1%	No	-	Human^e	Human^e
miR-455	17		>1%				>1%	No	-	-	-
miR-497	2	>5%	>5%	>5%	>5%			No	No	Human^e	Human^e
miR-497	2	>5%	>5%	>5%	>5%			No	No	Mouse^d,e	-
miR-497*	20	>5%	>5%					No	No	Mouse^d	-
miR-497*	20	>5%	>5%					No	No	-	-
miR-1251	6		>5%	>5%	>1%			No	No	Mouse^d,e	-
										-	-

Summary of the output from the miRNA editing detection pipeline, run with a 5% or 1% frequency cutoff. The two leftmost columns specify the miRNA ID and the position at which editing was observed. The following six columns show the detected editing events. For estimates of editing frequencies in individual samples, please refer to Figure 3 and Table S2 in Additional file 2. SNP data for human were taken from dbSNP [19], while opossum SNPs were investigated through Sanger sequencing (see Materials and methods). The two rightmost columns summarize whether the identified sites had previously been reported (‘known’) and experimentally validated (‘validated’) in human and mouse.
^a Blow et al. [10].
^b Kawahara et al. [11].
^c Kawahara et al. [12].
^d Chiang et al. [18].
^e Alon et al. [13].
^f Ekdahl et al. [14].
^g Vesely et al. [17].

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