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Figure 5 | Genome Biology

Figure 5

From: Genome-wide identification and functional analysis of Apobec-1-mediated C-to-U RNA editing in mouse small intestine and liver

Figure 5

Polysomal distribution of Apobec-1 mRNA editing targets. (A) Absorbance profile (A260) of fractions harvested from WT (green) and Apobec-1-/- (blue) mouse small intestine cytoplasmic extracts separated on sucrose gradients. Cytoplasmic extracts (two to five preparations) were prepared, each with three to four animals per genotype. (B) Sucrose gradient fractionation of apoB RNA from WT (green) and Apobec-1-/- small intestine cytoplasmic extracts (blue). ApoB RNA content in each fraction was analyzed in triplicate by quantitative PCR. Data were normalized to the expression of 18S mRNA and expressed as percentage of total apoB RNA. Data represent the mean of four to five separate isolations. (C-F) Polysomal distributions of Cyp2c65, Hpgd, Cyp2j6 and Ido1 RNAs, respectively, evaluated by quantitative PCR as described above. WT distribution (green), Apobec-1-/- distribution (blue).

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